Rhinomonas nottbecki n. sp. (Cryptomonadales) and Molecular Phylogeny of the Family Pyrenomonadaceae |
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| Authors: | Markus Majaneva Iina Remonen Janne‐Markus Rintala Ilya Belevich Anke Kremp Outi Setälä Eija Jokitalo Jaanika Blomster |
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| Institution: | 1. Tv?rminne Zoological Station, University of Helsinki, Hanko, 10900 Finland;2. Marine Centre, Finnish Environment Institute, Helsinki, 00560 Finland;3. Department of Environmental Sciences, University of Helsinki, Helsinki, 00014 Finland;4. M. Majaneva, Department of Environmental Sciences, University of Helsinki,;5. P.O. Box 65, 00014, Helsinki, Finland;6. Telephone number: +358 50 415 4644;7. FAX number: +358 019 280 122;8. e‐mail: markus.majaneva@gmail.com;9. Institute of Biotechnology, University of Helsinki, Helsinki, 00014 Finland |
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| Abstract: | The cryptomonad Rhinomonas nottbecki n. sp., isolated from the Baltic Sea, is described from live and fixed cells studied by light, scanning, and transmission electron microscopy together with sequences of the partial nucleus‐ and nucleomorph‐encoded 18S rRNA genes as well as the nucleus‐encoded ITS1, 5.8S, ITS2, and the 5′‐end of the 28S rRNA gene regions. The sequence analyses include comparison with 43 strains from the family Pyrenomonadaceae. Rhinomonas nottbecki cells are dorsoventrally flattened, obloid in shape; 10.0–17.2 μm long, 5.5–8.1 μm thick, and 4.4–8.8 μm wide. The inner periplast has roughly hexagonal plates. Rhinomonas nottbecki cells resemble those of Rhinomonas reticulata, but the nucleomorph 18S rRNA gene of R. nottbecki differs by 2% from that of R. reticulata, while the ITS region by 11%. The intraspecific variability in the ITS region of R. nottbecki is 5%. In addition, the predicted ITS2 secondary structures are different in R. nottbecki and R. reticulata. The family Pyrenomonadaceae includes three clades: Clade A, Clade B, and Clade C. All Rhinomonas sequences branched within the Clade C, while the genus Rhodomonas is paraphyletic. The analyses suggest that the genus Storeatula is an alternating morphotype of the genera Rhinomonas and Rhodomonas and that the family Pyrenomonadaceae includes some species that were described multiple times, as well as novel species. |
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| Keywords: | Cryptophyceae taxonomy ITS2 secondary structure
SSU
nucleomorph SBF‐SEM |
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