CadC activates pH-dependent expression of the Vibrio vulnificus cadBA operon at a distance through direct binding to an upstream region |
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| Authors: | Rhee Jee Eun Kim Kun-Soo Choi Sang Ho |
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| Affiliation: | Department of Food Science and Technology, School of Agricultural Biotechnology, Center for Agricultural Biomaterials, Seoul National University, South Korea. |
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| Abstract: | The Vibrio vulnificus cadBA genes were transcribed as a transcriptional operon by a single promoter, P(cadBA), which was activated by CadC in a pH-dependent manner. A direct interaction between CadC and the P(cadBA) DNA was demonstrated, and a CadC binding site centered at -233.5 was mapped by deletion analyses of P(cadBA) and confirmed by a DNase I protection assay. |
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