Age and Visual Experience-dependent Expression of NMDAR1 Splice Variants in Rat Retina |
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| Authors: | Georgia Manta Athanasios D Spathis Stavros Taraviras Elias D Kouvelas Adamantia Mitsacos |
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| Institution: | (1) Department of Physiology, School of Medicine, University of Patras, 26500 Patras, Greece;(2) Biomedical Research Foundation, Academy of Athens, 4 Soranou Efessiou, 11527 Athens, Greece; |
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| Abstract: | The N-methyl-D-aspartate receptor (NMDAR) is a key molecule mediating brain plasticity related processes. Knowing that alternative splicing
of the NMDAR1 (NR1) subunit offers molecular diversity to NMDAR, controls the forward trafficking of the NR1 protein and is
important for placing NMDA receptors at synapses, we investigated herein the postnatal developmental expression and the influence
of visual deprivation on NR1 subunit splice variants in rat retina. Real-time PCR was performed using oligonucleotide primers
specific for N- terminal (NR1a, NR1b) and C-terminal splice variants (NR1-1, NR1-2, NR1-3, NR1-4). The developmental profiles
of mRNA expression levels of all NR1 isoforms peaked at the end of the third week. Dark rearing led to reductions in both
N- and C-terminal NR1 variants in several developmental ages and a significant interaction between age and visual experience
was observed for NR1a, NR1-2 and NR1-4 expression. Our results have demonstrated a developmental and visual experience-dependent
regulation of NR1 splicing in rat retina. |
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