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Strategy for In Situ Detection of Natural Transformation-Based Horizontal Gene Transfer Events |
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| Authors: | Aurora Rizzi Alessandra Pontiroli Lorenzo Brusetti Sara Borin Claudia Sorlini Alessandro Abruzzese Gian Attilio Sacchi Timothy M. Vogel Pascal Simonet Marco Bazzicalupo Kaare Magne Nielsen Jean-Michel Monier Daniele Daffonchio |
| Abstract: | A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies. |
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