实时荧光定量PCR 法用于重组SIV-hPEDF注射液病毒颗粒数的检测 |
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引用本文: | 秦玺,李永红,杨琦,杨靖清,于雷,杨玉帅,徐莉,饶春明.实时荧光定量PCR 法用于重组SIV-hPEDF注射液病毒颗粒数的检测[J].现代生物医学进展,2015,15(8):1401-1405. |
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作者姓名: | 秦玺 李永红 杨琦 杨靖清 于雷 杨玉帅 徐莉 饶春明 |
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作者单位: | 中国食品药品检定研究院 |
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基金项目: | 863 计划项目(2012AA020805);国家科技重大专项项目(2012ZX09304010) |
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摘 要: | 目的:建立重组SIV-h PEDF注射液病毒颗粒数检测实时定量PCR检测方法。方法:在提取重组SIV-h PEDF注射液供试品基因组RNA后,采用实时定量PCR的方法,检测供试品中的病毒颗粒数。结果:经过3次实验,所得SIV-h PEDF RNA标准品标准曲线均线性良好,重组SIV-h PEDF注射液供试品中病毒颗粒数检测结果无显著性差异。结论:所建立的重组SIV-h PEDF注射液病毒颗粒数实时荧光定量PCR检测方法重复性好、灵敏、准确,可用于重组SIV-h PEDF注射液病毒颗粒数的检测。
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关 键 词: | 重组SIV-hPEDF注射液 实时定量PCR 病毒颗粒数 质量控制 |
Quantitation of Virus Particles in Recombinant SIV-hPEGF Injection by
Real-Time PCR |
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Abstract: | Objective:A Real-Time PCR method was developed to quantitatively determine the virus particles in recombinant
SIV-hPEGF injection.Methods:After extracting the genome RNA in recombinant SIV-hPEDF injection, Real-time PCR was used to
quantitatively determine the virus particles in test sample.Results:After 3 parallel experiments, the SIV-hPEDF RNA standard had a
good linear, and there was no significant difference in the detection results of virus particles in recombinant SIV-hPEGF injection.Conclusion:The established method is repeatable, sensitive and accurate, and can be applied in the determination of virus particles in
recombinant SIV-hPEGF injection. |
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Keywords: | Recombinant SIV-hPEGF Injection Real-time PCR Virus particles Quality control |
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