Biodegradation of methyl <Emphasis Type="Italic">tert</Emphasis>-butyl ether by newly identified soil microorganisms in a simple mineral solution |
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Authors: | Maria José Barberà Estíbaliz Mateo Rasa Monkaityte Magda Constantí |
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Institution: | 1.Departament d′Enginyeria Química,Universitat Rovira i Virgili,Tarragona,Spain |
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Abstract: | Methyl tert-butyl ether (MTBE) is a widely used fuel ether, which has become a soil and water contaminant. In this study, 12 microbial
strains were isolated from gasoline-contaminated soils and selected because of their capacity to grow in MTBE. The strains
were identified by 16S/ITS rDNA gene sequencing and screened for their ability to consume MTBE aerobically in a simple mineral
solution. Solid phase microoextraction and gas chromatography were used to detect MTBE degradation. High levels of MTBE biodegradation
were obtained using resting cells of the bacteria Achromobacter xylosoxidans MCM1/1 (78%), Enterobacter cloacae MCM2/1 (50%), and Ochrobactrum anthropi MCM5/1 (52%) and the fungus Exophiala dermatitidis MCM3/4 (14%). Our phylogenetic analysis clearly shows that bacterial MTBE biodegraders belong to the clade of Proteobacteria.
For further insight, MTBE-degrader strains were profiled by denaturing gel gradient electrophoresis (DGGE) of PCR-amplified
16S rRNA gene sequences. This approach could be used to analyse microbial community dynamics in bioremediation processes. |
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