| 逆境处理和DNA甲基化影响柑橘体细胞胚发生 |
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| 引用本文： | 郝玉金 邓秀新. 逆境处理和DNA甲基化影响柑橘体细胞胚发生[J]. Acta Botanica Sinica, 2002, 44(6): 673-677 |
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| 作者姓名： | 郝玉金 邓秀新 |
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| 作者单位： | 华中农业大学作物遗传改良国家重点实验室 武汉430070(郝玉金)，华中农业大学作物遗传改良国家重点实验室 武汉430070(邓秀新) |
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| 基金项目： | 国家自然科学基金 ( 39830 2 6 0 )~~ |
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| 摘 要： | 对 1 5种柑橘胚性愈伤组织进行体细胞胚诱导 ,发现逆境处理有利于体细胞胚发生 ,并可以恢复部分品种的体细胞胚发生能力。对具有和失去体细胞胚发生能力的两种纽荷尔脐橙 (CitrussinensisOsb .)愈伤组织进行随机扩增多态性DNA (RAPD)分析没有检测到带型的差异 ,而对它们的甲基化敏感扩增多态性 (MSAP)进行分析则发现两种愈伤组织间具有明显的DNA甲基化差异 ,具体细胞胚发生能力的愈伤组织的甲基化水平较失去体细胞胚发生能力的低
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| 关 键 词： | 干燥处理 低温处理 DNA甲基化 体细胞胚发生 RAPD MSAP |
Stress Treatments and DNA Methylation Affected the Somatic Embryogenesis of Citrus Callus |
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| HAO Yu_Jin ,DENG Xiu_Xin. Stress Treatments and DNA Methylation Affected the Somatic Embryogenesis of Citrus Callus[J]. , 2002, 44(6): 673-677 |
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| Authors: | HAO Yu_Jin DENG Xiu_Xin |
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| Affiliation: | HAO Yu_Jin *,DENG Xiu_Xin ** |
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| Abstract: | The evaluation on the callus embryogenesis capacity of 15 genotypes of citrus showed that stress treatments were conducive to somatic embryogenesis and could enhance the recovery of the missed capacity of embryogenesis for some genotypes. Randomly amplified polymorphic DNA (RAPD) and methylation sensitive amplified polymorphism (MSAP) analysis indicated that there existed significant differences in DNA methylation status between the callus capable of producing somatic embryoids and that which missed the embryogenesis capacity of the same genotype Newhall navel orange ( Citrus sinensis Osb. cv. Newhall). The DNA methylation level of the former was lower than that of the latter. However, RAPD profiles did not show any difference between these two kinds of callus. |
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| Keywords: | desiccation low_temperature treatment DNA methylation somatic embryogenesis RAPD MSAP |
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