Different structure of polytene chromosomes ofPhaseolus coccineus suspensors during early embryogenesis |
| |
Authors: | Anna Maria Tagliasacchi Laura Maria Costantina Forino Manuela Frediani Silvana Avanzi |
| |
Institution: | (1) Institute of Botany, The University, Via Luca Ghini 5, I-56100 Pisa, Italy |
| |
Abstract: | Summary The pattern of DNA and RNA puffs in pair VII of polytene chromosomes has been investigated in the suspensor ofPhaseolus coccineus during early embryo development. The pattern of3H-TdR and3H-U incorporation has been also detected. Collected data indicate that: 1. both heterochromatic regions, p11 and q(111+112), of chromosome pair VII, organize large DNA puffs; 2. DNA puffs of both regions are specific of different embryo differentiation steps; 3. a seasonal influence on the DNA puffing seems also to be present, as demonstrated by the comparison of the results collected in two different crops; 4. the incorporation experiment by3H-TdR evidences that not all DNA puffs show clustered labeling; 5. the RNA puffing of the two regions seems also to be specific of determined embryo stages. |
| |
Keywords: | DNA puffs RNA puffs Polytene chromosomes Labeling pattern Embryo suspensor |
本文献已被 SpringerLink 等数据库收录! |