Current methods for molecular typing of Campylobacter species |
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| Authors: | Eduardo N. Taboada Clifford G. Clark Emma L. Sproston Catherine D. Carrillo |
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| Affiliation: | 1. Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, PO Box 640, Township Rd. 9-1, Lethbridge, AB T1J 3Z4, Canada;2. National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington St., Winnipeg, Manitoba R3E 3R2, Canada;3. Bureau of Microbial Hazards, Health Canada, 251 Sir Frederick Banting Driveway, Ottawa, ON K1A 0C6, Canada;4. Canadian Food Inspection Agency, 960 Carling Avenue, Ottawa, ON K1A 0C6, Canada |
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| Abstract: | Campylobacter remains one of the most common bacterial causes of gastroenteritis worldwide. Tracking sources of this organism is challenging due to the large numbers of human cases, and the prevalence of this organism throughout the environment due to growth in a wide range of animal species. Many molecular subtyping methods have been developed to characterize Campylobacter species, but only a few are commonly used in molecular epidemiology studies. This review examines the applicability of these methods, as well as the role that emerging whole genome sequencing technologies will play in tracking sources of Campylobacter spp. infection. |
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| Keywords: | PFGE, pulsed field gel electrophoresis flaA-SVR, flaA short variable region sequence typing flaA-RFLP, flaA restriction fragment length polymorphism analysis MLST, multi-locus sequence typing eMLST, extended MLST RAPD, random amplification of polymorphic DNA WGS, whole genome sequencing NGS, next-generation sequencing MCGH, microarray comparative genomic hybridization CGF, comparative genomic fingerprinting SNPs, single nucleotide polymorphisms HRM, high-resolution melting analysis |
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