Differential recovery of bacterial and archaeal 16S rRNA genes from ruminal digesta in response to glycerol as cryoprotectant |
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| Authors: | Nest McKain Buğra Genc Timothy J. Snelling R. John Wallace |
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| Affiliation: | 1. Rowett Institute of Nutrition and Health, University of Aberdeen, Aberdeen, UK;2. Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey |
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| Abstract: | Bacteria and archaea in frozen (− 20 °C) ruminal digesta were analysed by qPCR and cloning/sequencing of 16S rRNA genes. Samples frozen with and without glycerol as cryoprotectant indicated a major loss of Bacteroidetes in unprotected samples, resulting in higher proportions of Firmicutes. Archaeal numbers and diversity were unaffected. |
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| Keywords: | Cryoprotection Glycerol Rumen Storage |
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