<Emphasis Type="SmallCaps">l</Emphasis>-Dopa decarboxylase expression profile in human cancer cells |
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Authors: | Ioanna?Chalatsa Eleftheria?Nikolouzou Emmanuel?G?Fragoulis Email author" target="_blank">Dido?VassilacopoulouEmail author |
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Institution: | (1) Department of Biochemistry and Molecular Biology, University of Athens, Panepistimiopolis, Zografou, 15701 Athens, Greece; |
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Abstract: | l-Dopa decarboxylase (DDC) catalyses the decarboxylation of l-Dopa. It has been shown that the DDC gene undergoes alternative splicing within its 5′-untranslated region (UTR), in a tissue-specific
manner, generating identical protein products. The employment of two alternative 5′UTRs is thought to be responsible for tissue-specific
expression of the human DDC mRNA. In this study, we focused on the investigation of the nature of the mRNA expression in human
cell lines of neural and non-neural origin. Our results show the expression of a neural-type DDC mRNA splice variant, lacking
exon 3 in all cell lines studied. Co-expression of the full length non-neural DDC mRNA and the neural-type DDC splice variant
lacking exon 3 was detected in all cell lines. The alternative DDC protein isoform, Alt-DDC, was detected in SH-SY5Y and HeLa
cells. Our findings suggest that the human DDC gene undergoes complex processing, leading to the formation of multiple mRNA
isoforms. The study of the significance of this phenomenon of multiple DDC mRNA isoforms could provide us with new information
leading to the elucidation of the complex biological pathways that the human enzyme is involved in. |
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