An Improved Protocol for the Preparation of Total Genomic DNA from Isolates of Yeast and Mould Using Whatman FTA Filter Papers |
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| Authors: | Andrew M. Borman Mark Fraser Christopher J. Linton Michael D. Palmer Elizabeth M. Johnson |
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| Affiliation: | (1) Mycology Reference Laboratory, Health Protection Agency, South-West Regional Laboratory, Bristol, UK;(2) HPA Mycology Reference Laboratory, Myrtle Road, Kingsdown, Bristol, BS2 8EL, UK |
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| Abstract: | ![]()
Here, we present a significantly improved version of our previously published method for the extraction of fungal genomic DNA from pure cultures using Whatman FTA® filter paper matrix technology. This modified protocol is extremely rapid, significantly more cost effective than our original method, and importantly, substantially reduces the problem of potential cross-contamination between sequential filters when employing FTA technology. |
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