Characterization of microRNAs from sheep (<Emphasis Type="Italic">Ovis aries</Emphasis>) using computational and experimental analyses |
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| Authors: | Xihui Sheng Xuemei Song Yan Yu Lili Niu Shangang Li Hongbin Li Caihong Wei Tao Liu Li Zhang Lixin Du |
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| Institution: | (1) National Center for Molecular Genetics and Breeding of Animal, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, People’s Republic of China;(2) College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA;(3) The Institute of Animal Husbandry and Veterinary Science, ZheJiang Academy Of Agricultural Sciences, Shiqiao Rd., Hangzhou, Zhejiang, 310021, People’s Republic of China;(4) Reproductive Medical Center, The Second Hospital Affiliated to Shandong University of Traditional Chinese Medicine, Jinan, 250001, People’s Republic of China;(5) Division of Applied Life Science, Graduate School of Gyeongsang National University, Jinju, 660-701, Korea;(6) Department of Laboratory Animal Sciences, School of Medicine, Shanghai Jiao Tong University, 200025 Shanghai, People’s Republic of China;(7) Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing, 100101, People’s Republic of China |
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| Abstract: | Ovis aries is one of the most important agricultural livestock for meat production, and also is an ideal model organism for biological
and comparative genomics studies. Many miRNAs have been reported for their important roles in developmental processes in various
animals, but there is limited information about O. aries miRNAs. In this study, combining a computational method based on expressed sequence tag (EST) analysis with experimental
identification based on small RNA cDNA library, we identified 31 miRNAs belong to 24 families in sheep, 2 of which were novel
miRNAs which had never been previously identified in any species. Especially, we cloned 12 miRNAs from the sheep skeletal
muscle, which were good candidate miRNAs to be studied about the miRNA-dependant regulated process of muscle development,
and we identified four pairs of miRNA/miRNA* and one pair of miRNA-3p/miRNA-5p from sheep EST sequences. Expression analysis
indicated that some miRNAs were expressed in a specific tissue, and the pair of miRNA-3p/miRNA-5p and one pair of miRNA/miRNA*
had a similar relative expression pattern in some tissues, respectively. Further, we predicted 120 potential target genes
of 31 oar-miRNAs on the 3′UTR of O. aries genes. Gene ontology analysis showed that most of these genes took part in the cellular process and metabolic process. Our
results enriched the O. aries miRNA database and provided useful information for investigating biological functions of miRNAs and miRNA* in sheep. |
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