| 植物小RNA荧光原位杂交实验方法 |
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| 引用本文： | 胡滨滨,薛治慧,张翠.植物小RNA荧光原位杂交实验方法[J].植物学报,2021,56(3):330-338. |
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| 作者姓名： | 胡滨滨 薛治慧 张翠 |
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| 作者单位： | 1中国科学院植物研究所, 中国科学院植物分子生理学重点实验室, 北京 1000932中国科学院大学, 北京 100049 |
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| 基金项目： | 中国科学院战略性先导科技专项(A类) No(XDA24010106-2);国家自然科学基金No(31900391) |
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| 摘 要： | 小RNA是对植物生长发育十分重要的一类小分子核苷酸，在多种生命过程以及胁迫响应中发挥重要调控作用。对小RNA的定位研究有助于揭示它们的功能，而小RNA荧光原位杂交(sRNA-FISH)是一种通过荧光检测技术对生物体内小RNA进行定性或半定量分析的技术，目前该技术已经在动物体内被广泛应用，而在植物体内的应用还比较少。该文...
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| 关 键 词： | 荧光原位杂交 小RNA 植物 |
| 收稿时间： | 2020-04-02 |
Protocols for Small RNA FISH in Plants |
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| Binbin Hu,Zhihui Xue,Cui Zhang.Protocols for Small RNA FISH in Plants[J].Bulletin of Botany,2021,56(3):330-338. |
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| Authors: | Binbin Hu Zhihui Xue Cui Zhang |
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| Institution: | 1Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China2University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | Small RNAs are a type of small nucleotide molecules that are essential for plant growth and development, playing a key role in variety of life processes and in response to stresses. Research on the location of small RNAs could discover their functions in plants. Small RNA FISH is a qualitative or semi-quantitative analysis of small RNA in organisms by fluorescence detection technology. At present, this technology has been widely used in animals, but it is still less applied in plants. This article introduces the specific operation procedures and attentions based on ultra-high resolution microscopy that combines locked nucleic acid (LNA) probe in situ hybridization with immunofluorescence. This protocol can be used to detect the expression and localization of small RNA in plant tissues. |
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| Keywords: | fluorescent in situ hybridization small RNA plants |
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