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Phenotypic and Molecular Assessment of Wheat Genotypes Tolerant to Leaf Blight,Rust and Blast Diseases
Authors:Md. Ashraful Alam  Milan Skalicky  Muhammad Rezaul Kabir  Md. Monwar Hossain  Md. Abdul Hakim  Md. Siddikun Nabi Mandal  Rabiul Islam  Md. Babul Anwar  Akbar Hossain  Fahmy Hassan  Amaal Mohammadein  Muhammad Aamir Iqbal  Marian Brestic  Mohammad Anwar Hossain  Khalid Rehman Hakeem  Ayman EL Sabagh
Abstract:Globally among biotic stresses, diseases like blight, rust and blast constitute prime constraints for reducing wheatproductivity especially in Bangladesh. For sustainable productivity, the development of disease-resistant lines andhigh yielding varieties is vital and necessary. This study was conducted using 122 advanced breeding lines ofwheat including 21 varieties developed by Bangladesh Wheat and Maize Research Institute (BAMRI) with aimsto identify genotypes having high yield potential and resistance to leaf blight, leaf rust and blast diseases. Thesegenotypes were evaluated for resistance against leaf blight and leaf rust at Dinajpur and wheat blast at Jashoreunder field condition. Out of 122 genotypes tested, 20 lines were selected as resistant to leaf blight based onthe area under the diseases progress curve (AUDPC) under both irrigated timely sown (ITS) and irrigated latesown (ILS) conditions. Forty-two genotypes were found completely free from leaf rust infection, 59 genotypeswere identified as resistant, and 13 genotypes were identified as moderately resistant to leaf rust. Eighteen genotypes were immune against wheat blast, 42 genotypes were categorized as resistant, and 26 genotypes were identified as moderately resistant to wheat blast. Molecular data revealed that the 16 genotypes showed a positive 2NSsegment among the 18 immune genotypes selected against wheat blast under field conditions. The genotypesBAW 1322, BAW 1295, and BAW 1203 can be used as earlier maturing genotypes and the genotypes BAW1372, BAW 1373, BAW 1297 and BAW 1364 can be used for lodging tolerant due to short plant height. The genotypes WMRI Gom 1, BAW 1349 and BAW 1350 can be selected for bold grain and the genotypes WMRI Gom 1,BAW 1297, BAW 1377 can be used as high yielder for optimum seeding condition but genotypes BAW 1377and BAW 1366 can be used for late sown condition. The selected resistant genotypes against specific diseases can be used in the further breeding program to develop wheat varieties having higher disease resistance andyield potential.
Keywords:Wheat blast  leaf blight  leaf rust  2NS marker  grain yield
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