| 分子生物学方法提高电活性微生物胞外电子传递效率的研究进展* |
| |
| 引用本文： | 邵映芝,车鉴,程驰,江志阳,薛闯. 分子生物学方法提高电活性微生物胞外电子传递效率的研究进展*[J]. 中国生物工程杂志, 2021, 41(6): 50-59. DOI: 10.13523/j.cb.2102020 |
| |
| 作者姓名： | 邵映芝 车鉴 程驰 江志阳 薛闯 |
| |
| 作者单位： | 1 大连理工大学生物工程学院 大连市合成生物学应用转化工程技术研究中心 大连 1160242 大连鑫玉龙海洋生物种业科技股份有限公司 大连 1162223 中国科学院沈阳应用生态研究所 沈阳 110016 |
| |
| 基金项目： | * 国家自然科学基金(21878035);国家自然科学基金(21808026);中国博士后面上基金(2019M661100) |
| |
| 摘 要： | 微生物细胞与电极之间的胞外电子传递效率是限制微生物电化学技术发展的关键因素,而分子生物学的发展为提高胞外电子传递效率带来了光明前景。从四种具有代表性的纯培养电活性微生物(奥奈达希瓦氏菌、铜绿假单胞菌、硫还原地杆菌和工程大肠杆菌)和混合培养电活性微生物出发,综述了利用分子生物学手段改造几种电活性微生物的研究成果,阐明了针对特异的电活性微生物,如何采取相应的分子生物学手段提高其胞外电子传递的效率,并展望了未来的研究方向。
|
| 关 键 词： | 胞外电子传递 电活性微生物 分子生物学 电活性生物膜 |
| 收稿时间： | 2021-02-22 |
Advances in Molecular Biological Methods to Improve Extracellular Electron Transport Efficiency of Electroactive Microorganisms |
| |
| SHAO Ying-zhi,CHE Jian,CHENG Chi,JIANG Zhi-yang,XUE Chuang. Advances in Molecular Biological Methods to Improve Extracellular Electron Transport Efficiency of Electroactive Microorganisms[J]. China Biotechnology, 2021, 41(6): 50-59. DOI: 10.13523/j.cb.2102020 |
| |
| Authors: | SHAO Ying-zhi CHE Jian CHENG Chi JIANG Zhi-yang XUE Chuang |
| |
| Abstract: | The efficiency of extracellular electron transfer between microbial cells and electrodes is a key factor limiting the development of microbial electrochemical technology, and the development of molecular biology has brought bright prospects for improving the efficiency of extracellular electron transfer. The results of engineering four representative electroactive microorganisms (Shewanella oneidensis MR-1, Pseudomonas aeruginosa, Geobacter sulfurreducens and engineered Escherichia coli) in pure culture and mixed culture by means of molecular biology are reviewed. How molecular biology methods adopt corresponding improvement strategies for different electroactive microorganisms are explained, and future research directions are prospected. |
| |
| Keywords: | Extracellular electron transfer Electroactive microorganism Molecular biology Electroactive biofilm |
|
| 点击此处可从《中国生物工程杂志》浏览原始摘要信息 |
|
点击此处可从《中国生物工程杂志》下载全文 |
