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Molecular Characterization of a Strain of Squash leaf curl China Virus from North India
Authors:R. Singh    S. K. Raj    V. Prasad
Affiliation:Authors' addresses: Molecular Plant Virology, National Botanical Research Institute, Lucknow 226 001, India;;Molecular Plant Virology Lab, Department of Botany, Lucknow University, Lucknow 226 007, India (correspondence to S. K. Raj. E-mail: )
Abstract:
A Begomovirus causing yellow vein mosaic disease of pumpkin (Cucurbita maxima L.) was characterized at molecular level by cloning and sequence analysis of its complete DNA‐A genome. The DNA‐A of the isolate contains 2758 nucleotides which encode six open reading frames (ORFs): AV1 and AV2 in the virion‐sense and AC1, AC2, AC3 and AC4 in the complementary‐sense. Based on the highest (96%) sequence identities and close phylogenetic relationships with Squash leaf curl China virus species, the Begomovirus was identified as strain of Squash leaf curl China virus. The presence of DNA‐B genome of the virus strain was also detected by dot blot hybridization test using DNA‐B specific probe.
Keywords:Squash leaf curl China virus    yellow vein mosaic disease of pumpkin    DNA-A    sequence identities    phylogenetic analysis    India
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