Production and partial characterization of a novel capsular polysaccharide KP-EPS produced by <Emphasis Type="Italic">Paenibacillus pabuli</Emphasis> strain ATSKP |
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Authors: | K P Pooja T S Chandra |
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Institution: | (1) Biochemistry Laboratory, Department of Chemistry, Indian Institute of Technology Madras, Chennai, 600 036, India;(2) Department of Biotechnology, Indian Institute of Technology Madras, Chennai, 600 036, India |
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Abstract: | The aerobic nitrogen fixing xylanolytic bacterium Paenibacillus pabuli strain ATSKP produces loosely attached capsular polysaccharide KP-EPS. On 0.5% birchwood xylan 70 ± 5.02 mg of KP-EPS was
produced per gram dry weight of cells by the fourth day of growth in the absence of combined nitrogen source at 30°C. It was
separated and purified using centrifugation, cold acetone precipitation and dialysis and is a sulfate containing heteropolymer
as revealed by FT-IR spectrometry and elemental analysis. CHN analysis revealed the presence of 37.50% carbon, 5.90% hydrogen
and 8.28% nitrogen in KP-EPS. Absence of phosphorus was confirmed by 31P NMR. ICP-OES analysis showed the presence of various metals in small concentrations. Specific binding with aniline blue
suggested the presence of (1,3)-β-d-glucan. Thermal gravimetric analysis and differential scanning calorimetric analysis confirmed its thermal stability as high
as 200°C. The EPS was not pseudo plastic and the viscosity was less than xanthan. The intrinsic viscosity did not reduce drastically
when dissolved in 0.1 M NaCl. |
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Keywords: | Paenibacillus pabuli strain ATSKP Capsular polysaccharide Nitrogen-fixing Xylanolytic |
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