Characterization of Phosphate-Solubilizing Fluorescent Pseudomonads from the Rhizosphere of Seabuckthorn Growing in the Cold Deserts of Himalayas |
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Authors: | Arvind Gulati Praveen Rahi Pratibha Vyas |
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Institution: | (1) Institute of Himalayan Bioresource Technology, P.O. Box No. 6, Palampur, HP, 176 061, India |
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Abstract: | Isolation and characterization of fluorescent pseudomonads with high phosphate-solubilizing ability is reported from the alkaline
and calcium-rich soils with low P availability in the cold desert region of Lahaul and Spiti in the trans-Himalayas of India.
Of 216 phosphate-solubilizing isolates, 12 exhibiting high solubilization of tricalcium phosphate (TCP) in NBRIP liquid culture
were identified as Pseudomonas
trivialis, P. poae, P. fluorescens, and Pseudomonas spp. on the basis of phenotypic features, whole-cell fatty acids methyl ester (FAME) profiles, and 16S rDNA sequencing. These
isolates also showed relatively high solubilization of North Carolina rock phosphate (NCRP) in comparison to the solubilization
of Mussoorie rock phosphate (MRP) and Udaipur rock phosphate (URP). The solubilization of phosphate substrates by P. trivialis and P. poae is reported for the first time. |
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