利用KASP标记筛选含rhg1和Rhg4位点的大豆抗病资源

大豆胞囊线虫(SCN,soybean cyst nematode)病是一种世界性大豆病害,培育抗SCN大豆品种是防治SCN的重要措施.本研究利用来自抗SCN主效位点rhg1和Rhg4的2个KASP标记,对487份大豆材料进行筛选,选择含有抗性位点且农艺性状优异的材料;通过室内接种大豆胞囊线虫2号、4号、5号生理小种和新...

Marker-Assisted Screening of Soybean Cyst Nematode Germplasms Harboring Resistance Loci rhg1 and Rhg4

Soybean cyst nematode(SCN,Heterodera glycines Ichinohe)is one of the most yield-limiting diseases of soybean worldwide.Releasing SCN-resistant cultivars are the primary basis for controlling SCN.Identification of parent lines showing major resistant loci and desirable agronomic characteristics are prerequisite for breeding resistant cultivars.In this study,a total of 487 soybean accessions were genotyped using two KASP markers that targeted to two resistance loci rhg1 and Rhg4.The accessions,which were identified harboring resistant alleles,were further subjected for resistance inoculated with race-2,race-4,race-5 and race-X12 in the greenhouse.Twenty accessions were characterized simultaneously harboring rhg1 and Rhg4 loci,while two accessions was found with the Rhg4 locus.Testing for SCN resistance suggested that one accession showed moderate resistant to three races and five accessions showed resistant or moderately resistant to two races.One accession was resistant and four accessions were moderately resistant to race-2.Two accessions showed moderately resistant to race-4.Four accessions showed resistant and fourteen moderately resistant to race-5.However,these accessions were turned out to be moderately susceptible or susceptible against race-X12.Collectively,by taking use of 487 soybean accessions this study identified 20 accessions that harbored two major resistant loci and showed desirable agronomic characteristics,might serve as elite parental lines in breeding for resistant cultivars via pyramiding rhg1 and Rhg4.