| MALDI-TOF-MS方法快速鉴定食品中空肠弯曲菌的技术研究 |
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| 引用本文： | 吴福平,吴瑜凡,崔思宇,邵景东.MALDI-TOF-MS方法快速鉴定食品中空肠弯曲菌的技术研究[J].工业微生物,2022,52(1):15-19. |
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| 作者姓名： | 吴福平 吴瑜凡 崔思宇 邵景东 |
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| 作者单位： | 张家港检验认证有限公司,张家港215600;张家港海关综合技术中心,张家港215600;华东理工大学分析测试中心,上海200237;常州经济开发区环境卫生管理处,常州213000;张家港海关综合技术中心,张家港215600 |
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| 基金项目： | 江苏省科技厅青年基金项目(BK20190235):基于CRISPR-CAS系统的空肠弯曲菌分型及其与毒力和耐药的相关性研究。 |
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| 摘 要： | 运用基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time of-flight mass spectrometry,MALDI-TOF-MS)技术快速鉴定食品中空肠弯曲菌.通过对该方法的样品前处理的选择、稳定性、特异性等方面进行研究,确定了方法...
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| 关 键 词： | 空肠弯曲菌 基质辅助激光解吸电离飞行时间质谱 快速鉴定 |
Rapid identification of Campylobacter jejuni in food by MALDI-TOF MS method |
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| WU Fuping,WU Yufan,CUI Siyu,SHAO Jingdong.Rapid identification of Campylobacter jejuni in food by MALDI-TOF MS method[J].Industrial Microbiology,2022,52(1):15-19. |
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| Authors: | WU Fuping WU Yufan CUI Siyu SHAO Jingdong |
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| Institution: | (Zhangjiagang inspection & Certification Co., Ltd, Zhangjiagang 215600;Comprehensive technology center of Zhangjiagang customs, Zhangjiagang 215600;Analysis and testing center of East China University of Technology, Shanghai 200237;Environmental Sanitation Management Office of Changzhou Economic Development Zone, Changzhou 213000) |
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| Abstract: | Matrix-assisted laser desorption/ionization time of-flight mass spectrometry(MALDI-TOF-MS)technology was used for rapid identification of Campylobacter jejuni in food.The feasibility of the method was determined by studying the selection,stability,and specificity of the sample pretreatment of this method.The results showed that the MALDI-TOF-MS identification method was accurate,rapid,and stable.Using this method,the relative strains of Campylobacter could be accurately and quickly distinguished,which were highly consistent with the VITEK biochemical identification results,and the accuracy and timeliness were greatly improved.The MALDI-TOF-MS method was suitable for rapid screening of Campylobacter jejuni in food. |
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| Keywords: | Campylobacter jejuni MALDI-TOF-MS rapid identification |
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