Identification of a nodD-like gene in Frankia by direct complementation of a Rhizobium nodD-mutant. |
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| Authors: | Li-mei Chen Yu-hai Cui Min Qin Yan-ling Wang Xue-liang Bai and Qing-sheng Ma |
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| Institution: | (1) Laboratory of Molecular Genetics, Guangxi Agricultural College, 530005 Nanning, China;(2) Present address: Shenyang Institute of Applied Ecology, Academia Sinica, China;(3) Present address: Bioengineering Department, Sichuan University, China |
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| Abstract: | Summary Clones from aFrankia At4 gene bank were pooled into groups and mass conjugated into anodD mutant ofRhizobium leguminosarum bv.viciae by triparental matings. When peas were inoculated with the pooled transconjugants, nodulation was observed. A plasmid, pAt2GX containingFrankia DNA, was isolated from bacteria recovered from these nodules. This plasmid was shown to complement anodD mutant ofR. leguminosarum bv.viciae. Thus pAt2GX contains aFrankia gene that is functionally equivalent tonodD ofR. leguminosarum bv.viciae. |
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| Keywords: | Frankia Nod Nodule Complementation Mutant |
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