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Comparison of DNA extraction methods for multiplex polymerase chain reaction |
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| Authors: | Triin Viltrop Kaarel Krjutškov Priit Palta Andres Metspalu |
| Institution: | a Department of Biotechnology, IMCB, University of Tartu, 51010 Tartu, Estonia b Estonian Biocentre, 51010 Tartu, Estonia c Department of Bioinformatics, IMCB, University of Tartu, 51010 Tartu, Estonia d Estonian Genome Center, University of Tartu, 50410 Tartu, Estonia |
| Abstract: | We compared six DNA extraction methods for obtaining DNA from whole blood and saliva for use in multiplex polymerase chain reaction (PCR) assays. The aim was to evaluate saliva sampling as an alternative to blood sampling to obtain DNA for molecular diagnostics, genetic genealogy, and research purposes. The DNA quantity, DNA purity (A260/280), PCR inhibition ratio, and mitochondrial DNA/genomic DNA ratio were measured to compare the extraction methods. The different extraction methods resulted in variable DNA quantity and purity, but there were no significant differences in the efficiency of multiplex PCR and oligomicroarray signals after single-base extension on the arrayed primer extension 2 (APEX-2). |
| Keywords: | Arrayed primer extension Multiplex PCR Saliva DNA |
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