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Mapping the myoglobin concentration, oxygenation, and optical pathlength in heart ex vivo using near-infrared imaging |
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| Authors: | Eugene Gussakovsky Yanmin Yang Olga Jilkina Valery Kupriyanov |
| Institution: | a National Research Council of Canada Institute for Biodiagnostics, Winnipeg, Manitoba, Canada R3B 1Y6 b Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada R3E 3P5 c Faculty of Dentistry, University of Manitoba, Winnipeg, Manitoba, Canada R3E 3P5 |
| Abstract: | A method that provides maps of absolute concentrations of oxygenated and deoxygenated myoglobin (Mb), its oxygenation, and its near-infrared (NIR) optical pathlength in cardiac tissue was developed. These parameters are available simultaneously. The method is based on NIR diffuse reflectance spectroscopic imaging and specific processing of the NIR images, which included a first derivative of the diffuse reflectance spectrum. Mb oxygenation, total Mb concentration, and NIR light pathlength were found to be in the range of 92%, 0.3 mM, and 12.5 mm, respectively, in beating isolated buffer-perfused and arrested pig hearts. The charge-coupled device camera enables sub-millimeter spatial resolution and spectroscopic imaging in 1.5 to 2.0 min. The technique is noninvasive and nondestructive. The equipment has no mechanical contact with the tissue of interest, leaving it undisturbed. |
| Keywords: | Myoglobin NIR light diffuse reflectance NIR spectroscopic imaging First derivative Optical pathlength Pig heart |
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