Development of enzyme linked immunosorbent assays to measure Bm86 antigen of Boophilus microplus (cattle tick) and to detect anti-Bm86 antibodies in serum samples |
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| Authors: | Triguero A. Blanco R. Machado H. Rodríguez M. de la Fuente J. |
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| Affiliation: | (1) Centro de Ingeniería Genética y Biotecnología, P.O.Box 83, Sancti Spiritus, Cuba;(2) Mammalian Cell Genetics Division. Centro de Ingeniería Genética y Biotecnología, P.O. Box 6162, Havana, Cuba |
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| Abstract: | ![]()
The immunization of cattle with the Boophilus microplus Bm86 antigen has been successful for the control of cattle tick infestations. To monitor the Bm86 production process and to measure the anti-Bm86 antibody titers in vaccinated cattle, mAb-based ELISA were developed and validated. The development of both immunological methods is essential to obtain a product with high quality and immunogenic properties and to monitor the immunological protection induced in vaccinated cattle against B. microplus. |
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| Keywords: | antibody ELISA Bm86 cattle tick Boophilus microplus |
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