Kinetics of in vivo bacteriochlorophyll fluorescence yield and the state of photosynthetic apparatus of purple bacteria |
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| Authors: | David Bina Radek Litvin Frantisek Vacha |
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| Institution: | (1) Biology Centre of AVCR, v.v.i, Institute of Plant Molecular Biology, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic;(2) Faculty of Science, University of South Bohemia, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic;(3) Institute of Physical Biology, University of South Bohemia, Zamek 136, 373 33, Nove Hrady, Czech Republic |
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| Abstract: | The light-induced electron transport in purple bacterium Rhodobacter sphaeroides was studied in vivo by means of kinetic difference absorption spectroscopy and kinetics of bacteriochlorophyll fluorescence
yield. Measurements of redox state of the oxidised primary donor and cytochrome c and the membrane potential revealed a complex pattern of changes of the electron flow. Effects of the membrane potential
on the fluorescence yield were also analysed, and a model for the fluorescence induction curve is presented. The data indicate
substantial positive effect of the membrane potential on the fluorescence emission in vivo. Moreover, light-induced changes
in light scattering were observed, which suggests occurrence of structural changes on the level of the photosynthetic membrane. |
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| Keywords: | Photosynthesis Fluorescence induction Rhodobacter sphaeroides Membrane potential Light scattering Absorbance changes |
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