Cloning and analysis of the inulinase gene from Kluyveromyces cicerisporus CBS4857 |
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| Authors: | Wen Tieqiao Liu Feng Huo Keke Li Yu-Yang |
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| Affiliation: | (1) Institute of Genetics, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, 200433, People's Republic of China;(2) Present address: School of Life Sciences, Shanghai University, Shanghai, 200436, People's Republic of China |
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| Abstract: | A highly expressed inulinase gene, KcINU1 was cloned and sequenced from Kluyveromyces cicerisporus CBS4857 a strain which secrets high levels of inulinase into the growth medium. The result of DNA sequencing showed that KcINU1 contained a 1665 bp ORF, coding for a 555 amino acid protein, in which a 23 amino acid signal peptide was included. The sequence has the GenBank Accession no. AF 178979. The analysis of conserved domain in the ORF indicated there was a consensus sequence about 470 amino acids long. The 0.7 Kb promoter and 0.9 Kb terminator were also cloned and sequenced. |
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| Keywords: | Gene cloning inulinase KcINU1 K. cicerisporus promoter signal peptide |
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