A semiautomated fluorometric determination of 5-hydroxyindoles in the nanogram range |
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Authors: | J Korf H H Schutte K Venema |
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Institution: | Department of Biological Psychiatry, Groningen University, Oostersingel 59, Groningen, The Netherlands |
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Abstract: | Automated determinations of 5-hydroxytryptamine and its main metabolite, 5-hydroxyindoleacetic acid, have been described (Technicon autoanalyzer). The determinations are based on an extraction procedure from deproteinized tissue extracts or cerebrospinal fluid by means of butanolheptane mixtures. The indoles are transferred from the organic phase to a water phase and determined fluormetrically with the cysteine-o-phthaldialdehyde method. The method is highly sensitive: solutions containing 1 ng/ml can be reproducibly determined. Twenty samples per hour can be passed through the system. The determination of both 5-hydroxyindoles is performed with the same manifold. |
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Keywords: | Requests to Mr K Venema Dept Biol Psych Oostersingel 59 Groningen The Netherlands |
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