| 乳液法静电纺丝PLGA组织工程缓释纤维的研究 |
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| 引用本文： | 高小寒,魏蓓蓓,田玲玲,刘一浓,秦明杰,胡振华,吴飞.乳液法静电纺丝PLGA组织工程缓释纤维的研究[J].生物磁学,2011(12):2224-2226. |
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| 作者姓名： | 高小寒 魏蓓蓓 田玲玲 刘一浓 秦明杰 胡振华 吴飞 |
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| 作者单位： | [1]上海交通大学药学院,上海200240 [2]东华大学纺织学院,上海201620 |
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| 基金项目： | “国家重大新药创制”针对生物技术药物的相关给药技术平台（2009ZX09310-007） |
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| 摘 要： | 目的：研究Dextran对蛋白药物的释放影响。方法：将模型蛋白BSA溶解于多糖溶液中,通过W/O乳液法静电纺丝制备缓释纤维。采用MicroBCA法测定该纤维体外释放行为,采用SEC-HPLC检测制备前后蛋白的聚集程度,并与不含多糖的BSA纤维做对照。结果：添加Dextran以后蛋白的包封率由52.68%提高到63.92%,第一天突释不大于药物载量的15%,对蛋白单体的保持达到85%以上。结论：Dextran可以改善一般组织工程纤维中蛋白药物的释放,提高蛋白药物在制剂、贮存、释放过程中的稳定性,增加纤维的载药量。
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| 关 键 词： | 乳液法 静电纺丝 PLGA 组织工程 葡聚糖 |
Encapsulation of Proteins in PLGA fibers by Emulsion Electrospinning |
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| GAO Xiao-han,WEI Bei-bei,TIAN Ling-ling,LIU Yi-nong,QIN Ming-jie,HU Zhen-hua,WU Fei.Encapsulation of Proteins in PLGA fibers by Emulsion Electrospinning[J].Biomagnetism,2011(12):2224-2226. |
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| Authors: | GAO Xiao-han WEI Bei-bei TIAN Ling-ling LIU Yi-nong QIN Ming-jie HU Zhen-hua WU Fei |
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| Institution: | 1 Shanghai Jiao Tong University School of Pharmacy,Shanghai 200240;2 Dong Hua University College of Textiles,Shanghai 201620) |
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| Abstract: | Objective：To investigate the effect of dextran on the releasing behavior of protein drug.Methods：BSA was dissolved in dextran solution,then the fibers was prepared by W/O emulsion electrospinning.The release behavior of the fibers in vitro was detected by MicroBCA method and BSA aggregation was determined by SEC-HPLC.The results were compared with the BSA fiber without dextran.Results：Drug loading for the experiment group reached 63.92 % while control group was only 52.68 %,with first day burst release less than 15 %,BSA monomer preserved more than 85 %.Conclusion：Dextran can improve release behavior of tissue engineering fibers,increase drug loading and drug stability during preparation,storation and release process. |
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| Keywords: | Emulsion Electrospinning Poly（lactic-co-glycolic acid） Tissue Engineering Dextran |
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