测定糖尿病患者血清LDL-C水平的方法学比较

目的：比较不同方法检测糖尿病患者血清LDL-C水平,为临床诊疗提供准确可行的检验方法。方法：采用沉淀法、匀相法、电泳法及超速离心法对233例糖尿病患者和102例健康人群的血清LDL-C水平进行测定,比较各方法之间的相关性,同时分析导致结果差异的因素。结果：四种方法检测健康人群LDL-C水平,结果间无统计学差异（P〉0.05）;糖尿病组,当TG≤2.26mmol/L时,四种方法检测LDL-C结果间相关性良好。高胆红素、血红蛋白、高TG及乳糜等干扰因素存在时,与其他方法相比,电泳法和超速离心法检测血清LDL-C结果受影响较小（P〉0.05）。结论：超速离心法虽耗时、价格贵,但仍为检测LDL-C的经典方法,电泳法受高胆红素、血红蛋白、高三酰甘油等干扰因素的影响相对较小,适用于糖尿病合并高血脂患者血清LDL-C水平检测。

Comparison of Methods in Determining Serum LDL-C in Diabetic Patients

Objective： To compare different methods of detection of serum LDL-C in diabetic patients, provide accuate and feasible test methods for clinical diagnosis and treatment. Methods： The serum lipid of 233 patients with diabetes mellitus and 102 normal healthy population were measured by precipitation, homogeneous, electrophoresis and ultracentrifugation methods, comparing the correlation among the methods, and analyzing possible factors effecting results difference. Results： Results of serum LDL-C of healthy population by four methods showed no significant difference（P0.05）; In the group of diabetic patients, TG≤ 2.26mmol/L, the results of LDL-C were no significant difference among four methods（P0.05）. When existing some interference 1 factors such as high bilirulin、hemoglobin and high triglyceride in serum, the results of LDL-C by electrophoresis and ultracentrifugation showed no changes comparing other methods （P0.05）. Conclusion： Although ultracentrifugation methods needs more and high price , it is still classic methods for LDL-C testing, electrophoresis were more suitable for lipids detection of diabetic patients with coronary heart disease because of relatively small effec- tion by high bilirulin、hemoglobin and high triglyceride.