胃转流术后远端肠黏膜形态及生长因子表达的变化

目的：探讨胃转流术后远端肠管黏膜的适应性变化及生长因子的表达情况。方法：将8周龄的Wistar大鼠随机分为对照组、假手术组和胃转流术组，术后8周取吻合口远端肠管行常规病理切片检查，测量肠黏膜厚度和绒毛高度，采用免疫荧光法检测肠粘膜中表皮生长因子（EGF）和胰岛素样生长因子-1（IGF—1）的表达。结果：胃转流术组黏膜厚度（672±39与500±31um，P〈0．01）和绒毛高度（445±19与342±15um，P〈0．01）均显著高于假手术组（P〈0．01），而对照组和假手术组间均无显著差异。与假手术组比较，胃转流术组肠粘膜中EGF和IGF-1的表达显著升高（P〈0．01），而对照组和假手术组间无显著差异。结论：胃转流术后远端肠管粘膜发生适应性增生，同时伴随着EGF和IGF—1表达水平的升高。

Changes in Morphology of Intestinal Mucosa and Expressions of Growth Factors after Gastric Bypass Surgery in Rats

Objective： To investigate the changes in the morphology of intestinal mucosa and the expressions of growth factors after gastric bypass surgery （GBP）. Methods： Wistar rats （eight-week old） were divided into three groups： control group, sham group, and GBP group. The distal bowel was collected after 8 weeks following the surgery. The mucosal thickness and villus height were measured by histological analysis. The expressions of epidermal growth factor （EGF） and insulin-like growth factor 1 （IGF-1） were detected by immunofluorescence. Results： The mucosal thickness （672 ± 39 vs. 500 ± 31um, P〈0.01） and villus height （445 ± 19 vs. 342 ± 15u m, P〈0.01） in GBP group were significantly higher than sham group. In addition, the mucosal thickness and villus height were similar between control group and sham group. Compared with sham group, the expressions of EGF and IGF-1 were significantly increased （both P〈0.01）. Additionally, the expressions of EGF and IGF-1 were similar between control group and sham group. Conclusion： Interstinal mucosal adaptation occurred in distal bowel, which is associated with increased expressions of EGF and IGF-1.