miR-143通过抑制PTN促进人骨髓间充质干细胞成脂分化

目的：研究miR-143调控人骨髓问充质干细胞（hMSCs）成脂分化的新机理。方法：将NC、miR-143、siPTN、miR-143i转入hMSCs中，诱导成脂分化，检测miR-143对成脂分化的影响。经miRNA靶点分析软件Findtar预测出miR-143在人多效生长因子（hPTN）的3＇-UTR端有靶点。RT-PCR、westernblot研究mjR-143与hPTN的关系。构建hPTN3＇-UTR靶位点荧光检测质粒prltk-PTN及其突变质粒prltk-m，验证miR-143是否在人PTN3＇-UTR上有靶点。结果：miR-143促进hMSCs成脂分化，抑制hPTN的mRNA和蛋白表达水平。荧光报告实验证实miR-143在人PTN的3＇-UTR上有靶点。结论：miR-143通过与hPTN3I-UTR上的靶点相结合而抑制hPTN的表达，从而促进了hMSCs成脂分化进程。

miR-143 Accelerates hMSCs Adipogenesis by Suppressing the Expresssion of PTN

Objective： To investigate the mechanism ofmiR-143 in the regulation of hMSCs adipogenesis. Methods： hMSCs were transfected with NC/miR-143/siPTN/miR-143i and induced into adipogenic differentiation to detect the effect ofmiR-143 on adipogenesis. The miR-143 target in 3＇-UTR of hPTN was predicted by Findtar-soflware of miRNA. The relationship between miR-143 and hPTN was researched by the methods of RT-PCR and western blot. The target of miR-143 predicted in hPTN 3＇-UTR was verified by luciferase expression of prltk-PTN plasmid inserted with hPTN 3＇-UTR segment and prltk-m plasmid of mutant target. Results： miR-143 promoted adipogenic differentiation of hMSCs, and suppressed the expression level of hPTN mRNA and protein. The experiment of luciferase demonstrated that there was miR-143 target in hPTN 3＇-UTR. Conclusion： miR-143 suppressed the expression of hPTN by combining with target in hPTN 3＇-UTR to accelerate the process ofhMSCs adipogenesis.