姜黄素对糖尿病大鼠疼痛过敏的影响

目的：神经病理性痛是糖尿病最常见的并发症之一，本课题旨在探讨姜黄素对糖尿病大鼠痛觉过敏的影响及其分子机制。方法：30只雄性SD大鼠随机分为对照组、糖尿病组和姜黄素治疗纽，模型纽和姜黄素治疗组利用腹腔注射链脲佐菌素（Streptozotocin，STZ）制备大鼠糖尿病模型，定期检测大鼠血糖、饮食、体重等变化，治疗组于STZ注射2wk后定期灌服姜黄素，分别在2wk和4wk后检测各组大鼠热痛敏和机械痛敏反应，在第4wk利用ELISA分别检测各组大鼠脊髓背角TNF-α表达变化。结果：STZ注射组大鼠2周后出现血糖〉14mol／L，并且该模型具有高血糖、体重增长缓慢、多饮多食多尿的特点，符合Ⅰ型糖尿病特征，痛行为测试结果显示糖尿病大鼠出现痛觉过敏，经过给予姜黄素灌服治疗后，痛觉过敏有所减轻，ELISA分析结果表明糖尿病大鼠脊髓背角TNF-α表达升高，经过姜黄素治疗后TNF-α表达有所下降。结论：成功制备STZ-型糖尿病大鼠模型，经过姜黄素治疗可以减轻糖尿病引起的疼痛过敏，姜黄素对糖尿病疼痛的治疗作用可能是通过降低大鼠脊髓背角TNF-α表达实现的。

Effect of Curcumin on Hyperalgesia of Diabetic Rats

Objective： The purpose of the study was to investigate the effects of curcumin on hyperalgesia of STZ-induced diabetic rats. Methods： 30 male SD rats were randomly divided into three groups：control group （CN）, diabetic mellitus group （DM） and curcumin therapy group （DM＋Cur）. Streptozotocin （STZ） prepared in citrate buffer was injected intraperitoneally to induce DM. DM＋Cur group were treated with 100 mg/kg curcumin everyday. Mechanical withdrawal threshold （MWT） and thermal withdrawal latency （TWL）of the rats were measured. The expressin of TNF-αin the spinal dorsal horn of rats was tested by ELISA. Results： The DM group and DM＋Cur group rats displayed hyperglycosemia, weight loss, polyphagia, polydipsia and polyuria. The MWT and TWL of DM group rats decreased compared with CN group rats. After treated with curcumin, both MWT and TWL of DM＋Cur group rats increased. The expression of TNF-αin the spinal dorsal horn of diabetic rats was higher than that of CN group. After treated with curcumin, the level of TNF-αwas reduced. Conclusions： Curcumin can and relieve diabetic neuropathic pain and reduce the expression of TNF-αin spinal cord dorsal horn of STZ inducing diabetic rats. The expression of TNF-αin the spinal cord dorsal horn may participate diabetic pain.