p53、BCL-2、Caspase－3等凋亡相关蛋白在大鼠脑损伤中的表达及意义

目的：检测脑外伤大鼠中p53、bcl-2及caspase-3表达，并分析其与脑外伤之间的关系，为脑损伤患者预后提供部分参数依据。方法：建立脑外伤大鼠实验动物模型，用免疫组化方法检测p53、Bcl-2和Caspase-3的表达。结果：脑创伤后在创伤周围区的神经元会发生凋亡，凋亡的发生可能与p53、bcl-2、caspase-3等基因及蛋白的调节有关。结论：脑创伤后在创伤周围区的神经元会发生凋亡，凋亡的发生可能与p53、bcl-2、caspase-3等基因及蛋白的调节有关。

Expression of p53, Bcl-2 and Caspase-3 in Rats with Brain Injury

Objective： To detect the expression of p53 ,Bcl-2 and Caspase-3 in brain injury rat and analyze its relationship with brain damage, to provide some parameters according for the prognosis of patients with brain damage. Methods： Establish brain injury rat experimental animal model, detected the expression of p53, Bcl-2 and Caspase-3 by immunohistochemistry. Results： The immunohisto-chemical results in brain injury rat experimental animal model： the brain injury model group compared with the normal group and sham operation group, p53 and Caspase-3 expression increased significantly, Bcl-2 expression dropped significantly, differences were statistically significant （P〈0.01）. Conclusion： After brain trauma, apoptosis of the neurons around the trauma will happen and it is possibly concerned with the regulation of genes and proteins p53, BCL-2 or caspase-3.