1,25二羟基维生素D_3对兔角膜碱烧伤朗格罕氏细胞影响

目的：研究1,25二羟基维生素D3（骨化三醇）对兔角膜碱烧伤后角膜朗格罕氏细胞分布的影响,并初步探讨其作用机制。方法：在兔角膜制作碱烧伤模型,然后实验组局部和全身给予1,25二羟基维生素D3,分别在第3,7,21天时对正常组,实验组和对照组家兔行角膜共聚焦显微镜,HE染色观察角膜病理改变。结果：正常组角膜中央在三个时间点均未检测出朗格罕氏细胞。实验组和对照组碱烧伤后3、7天角膜中央出现朗格罕氏细胞,对照组密度高于实验组（p〈0.05）;碱烧伤后21天两组朗格罕氏细胞密度相近（p〉0.05）。实验组炎性反应程度在第7,21天时轻于对照组。结论：1,25二羟基维生素D3能够在兔角膜碱烧伤早期抑制朗格罕氏细胞的向心性迁移,并且能在一定程度上抑制炎性反应。

Role of 1,25-dihydroxyvitamin D_3 on Langerhans cell in rabbitcorneal alkali burn

Objective：To investigate the role of 1,25-dihydroxyvitamin D3（calcitriol） on Langerhans cell in rabbit corneal alkali burn and preliminary study its mechanism action.Methods：The models of corneal alkali burn were induced in rabbit,then administer the experimental group 1,25-dihydroxyvitamin D3 in local and general way.Corneas of normal group,experimental group and control group were examined at day 3,7 and 21,analyzed by corneal confocal microscopy and H-E staining.Results：None Langerhans cell was found in the corneas of normal group on all three days.Less Langerhans cells were found in the experimental group than in the control group on day 3 and 7（p0.05）.Quantity of Langerhans cells in both experiment group and control group was no difference on the day 21（p0.05）.The inflammatory reaction degree of experimental group is slighter than the control group on the day 7 and 21.Conclusions：1,25-dihydroxyvitamin D3 can suppress Langerhans cell transfer centrality and provide a way to suppress the inflammatory reaction in prophase of rabbit corneal alkali burn.