染料木素对铅诱导的PC12细胞毒性的抑制效应研究

目的：探讨染料木素对铅诱导的细胞毒性的影响。方法：PC12细胞分为对照组、染铅组、染料木素组以及铅加染料木素组;MTT实验检测细胞活力的改变,流式细胞仪检测细胞凋亡水平的变化,荧光探针检测线粒体形态的改变,Western blot方法检测线粒体融合分裂相关蛋白表达水平的变化。结果：铅可诱导PC12细胞活力的下降以及细胞凋亡率的显著增高,染料木素可抑制铅的这些毒性效应。与此同时,铅可诱导线粒体形态的损伤性改变,线粒体融合减少,分裂增多;而加入染料木素之后,线粒体损伤程度显著下降,线粒体分裂减少,融合增多。此外,线粒体融合相关蛋白Mfn2的水平在铅暴露后显著下降,而线粒体分裂相关蛋白Drp1的水平在铅暴露后显著升高,染料木素干预后均有所恢复。结论：染料木素可抑制铅诱导的PC12细胞毒性,其作用可能与其对线粒体融合分裂过程的干预有关。

The Inhibitory Effect of Genistein on Lead-induced Cytotoxicity in PC12 Cells

Objective：To investigate the effect of genistein on lead-induced cytotoxicity in vitro.Methods：PC12 cells were divided into four groups：control,lead,genistein,and genistein＋lead.MTT method was used to detect the cell viability;Flow cytometry method was used to detect the apoptosis;A fluorescent probe was used to determine mitochondrial morphological changes;Western blot was used to detect the expression of mitochondrial fusion and fission-related proteins.Results：The exposure to lead caused the decrease of cell viability and the increase of apoptosis,both of which can be inhibited by genistein.And lead exposure induced the morphological changes of mitochondria,which is characterized by the decrease of mitochondrial fusion and the increase of mitochondrial fission,which also can be reversed by the supplement of genistein.Furthermore,the lead exposure resulted in the decreasing expression of mitochondrial fusion-related protein Mfn2 and the increasing expression of mitochondrial fission-related protein Drp1,both of which can be relieved with the supplement of genistein.Conclusion：Genistein can inhibit lead-induced cytotoxicity in PC12 cells,which can be relevant to its effects on mitochondrial fusion and fission.