Evaluation of antioxidant properties and total phenolic contents of some strains of microalgae |
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Authors: | Mannan Hajimahmoodi Mohammad Ali Faramarzi Najmeh Mohammadi Neda Soltani Mohammad Reza Oveisi Nastaran Nafissi-Varcheh |
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Institution: | (1) Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, 14174, Iran;(2) Department of Pharmaceutical Biotechnology; Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, 14174, Iran;(3) Department of Medical Biotechnology, Faculty of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, 14174, Iran;(4) Department of Biology, ACECR, Research Institute of Applied Science, Tehran, Iran;(5) Department of Pharmaceutical Biotechnology, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, P.O. Box 14155-6153, Tehran, Iran; |
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Abstract: | Antioxidant activities of both cells and extracellular substances were evaluated in 12 soil-isolated strains of microalgae
according to FRAP and DPPH-HPLC assays. Their total phenolic contents were also determined by Folin–Ciocalteu method. Extractions
were performed with hexane, ethyl acetate, and water. The results of FRAP assay showed that algal cells contained considerable
amounts of antioxidants from 0.56 ± 0.06 to 31.06 ± 4.00 μmol Trolox g−1 for Microchaete tenera hexane extract and Chlorella vulgaris water extract, respectively. In water fractions of extracellular substances, the antioxidants were from 1.30 ± 0.15 μmol
Trolox g−1 for Fischerella musicola to 73.20 ± 0.16 μmol Trolox g−1 for Fischerella ambigua. Also, DPPH-HPLC assay represented high antioxidant potential of water fractions. The measured radical-scavenging activities
of the studied microalgae were at least 0.15 ± 0.02 in Nostoc ellipsosporum cell mass to a maximum of 109.02 ± 8.25 in C. vulgaris extracellular substance. The amount of total phenolic contents varied in different strains of microalgae and ranged from
zero in hexane extract to 19.15 ± 0.04 mg GAE g−1 in C. vulgaris extracellular water fraction. Significant correlation coefficients between two measured parameters indicated that phenolic
compounds were a major contributor to the microalgal antioxidant capacities. |
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