Estimation of specific growth rate from agitation speed in DO-stat culture |
| |
Authors: | Tae Ho Lee Yong Keun Chang Bong Hyun Chung |
| |
Institution: | (1) Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 305-701 Taejon, Korea;(2) Korea Research Institute of Biotechnology and Bioengineering, 305-600 Taejon, Korea |
| |
Abstract: | Summary A simple and effective method to estimate the specific growth rate estimation has been developed based on the observation of time changes in the agitation speed in dissolved oxygen(DO)-stat cultures of Brevibacterium ketoglutamicum. The estimation was compared with that using carbon dioxide evolution rate (CER). Estimated values of specific growth rates by both methods agreed well with the data directly calculated from cell concentration change although the use of agitation speed gave a slightly better result than CER.Nomenclature
CER
Carbon dioxide evolution rate (mmol/sec)
-
OUR
Oxygen uptake rate (mmol/sec)
-
OTR
Oxygen transfer rate (mmol/sec)
-
RPM
Agitation speed (rev./min)
-
C*
Saturated dissolved oxygen concentration (mmol/L)
-
Dissolved oxygen concentration (mmol/L)
-
k
Time index
-
k
L
a'
Mass transfer coefficient (sec-1)
-
Y
X/O2
Cellular yield based on oxygen consumed (g-cell/mmol O2)
-
Specific growth rate (hr-1)
-
Constant
-
t
Fermentation time
-
t
Sampling time for RPM and CER measurements |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |