Primary structure of an N-linked sugar chain derived from glucoamylase of Rhizopus niveus |
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| Authors: | K Takegawa N Kawasaki S Iwahara K Yamamoto T Tochikura B Mikami Y Morita |
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| Institution: | Department of Bioresource Science, Kagawa University, Japan. |
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| Abstract: | The primary structure of the N-linked sugar chain of Rhizopus niveus glucoamylase (major component) was investigated. The carbohydrate moiety was released from the polypeptide backbone by Flavobacterium sp. endo-beta-N-acetylglucosaminidase digestion. Studies using the method of exoglycosidase digestion of the fluorescent pyridylamino derivative, gel-permeation chromatography on Bio-Gel P-4 and 400-MHz 1H-NMR spectroscopy revealed that the most abundant structure is (Man)8-GlcNac-ol. |
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