Genetic variation for in vitro sesame pollen germination and tube growth |
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Authors: | P L Pfahler M J Pereira R D Barnett |
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Institution: | (1) Agronomy Department, 304 Newell Hall, Box 110500, University of Florida, Gainesville, FL 32611-0500, USA, US |
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Abstract: |
In vitro pollen germination and tube length studies are valuable in elucidating mechanisms (germination capacity and rate, tube growth
rate) possibly associated with genetic differences in male transmission. On each of two collection dates, the percentage germination
and tube length of the binucleate pollen grains from five diverse sesame (Sesamum indicum L.) genotypes were determined at eight times (30, 60, 90, 120, 150, 180, 240, 300 min) after inoculation on a semisolid medium
containing 10% (100 g l-1) sucrose (C12H22O11), 0.4% (4 g l-1) purified agar (Fisher Lot 914409), 0.1% (1 g l-1) calcium nitrate Ca(NO3)2 ⋅ 4H2O] and 0.01% (100 mg l-1) boric acid (H3BO3). Before heating, the pH of the medium was adjusted to 7.0 with a 0.1 N potassium hydroxide (KOH) solution. Over the five genotypes, 5% germination was found 30 min after inoculation and a maximum
of 37% germination 120 min after inoculation with no significant changes thereafter. As indicated by the highly significant
genotype×time after inoculation interaction, the genotypes differed in the time at which germination was initiated and maximum
germination attained. Over all five genotypes, the tube length was 91 μm 30 min after inoculation, reaching a maximum of 1000 μm
300 min after inoculation. As shown by the highly significant genotype×time after inoculation interaction, the genotypes differed
in the time at which tube length was observed and the maximum tube length was attained. Little or no relationship between
percent germination and tube length was observed among the genotypes. For both percent germination and tube length, the statistical
significance of collection date and its interactions with genotype and time after inoculation indicated that environment in
the form of collection date was also an influencing factor. These results indicated that genetic differences among genotypes
were present for in vitro germination capacity, germination rate and tube growth rate and that these factors singly or in combination could alter male
transmission of genetic elements.
Received: 5 February 1997 / Accepted: 23 June 1997 |
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Keywords: | In vitro pollen germination Sesame Sesamum indicum L Male transmission |
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