Plant regeneration from shoot apex explants of foxtail millet |
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Authors: | Pedro Osuna-Avila Alejandro Nava-Cedillo Alba E Jofre-Garfias José Luis Cabrera-Ponce |
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Institution: | (1) Central Camionera, CIGA ITA 20, Apdo. Postal 1439, 20270 Aguascalientes, Ags., México;(2) Depto. de Ingeniería Genética de Plantas, CINVESTAV-IPN, Apdo. Postal 629, 36500 Irapuato, Gto., México |
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Abstract: | Green and etiolated shoot apices of foxtail millet (Setaria italica L.) cv. Nese 2A were cultured on Murashige and Skoog medium with four concentrations of 2,4-dichlorophenoxyacetic acid or 2,4,5-trichlorophenoxyacetic acid. In all treatments, embryogenic calli capable of plant regeneration were induced after ten weeks in culture. Calli induced on 2 mg l-1 of 2,4-d from green apices gave a higher rate of plant regeneration in comparison with etiolated apices on the other treatments. Plant regeneration was obtained from one year-old cultures. Regenerated plants were successfully established in soil, reached maturity and produced seeds.Abbreviations 2,4-d
2,4-dichlorophenoxyacetic acid
- EC
embryogenic calli
- NE
nonembryogenic calli
- 2,4,5-T
2,4,5-trichlorophenoxyacetic acid |
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Keywords: | Setaria italica |
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