pIF21, a versatile donor of transposons Tn1, Tn5 and Tn7 for tagging and mobilizing cryptic and non-selectable plasmids |
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| Authors: | IM Feavers A J Fife CR Harwood † |
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| Institution: | Department of Microbiology, Medical School, Newcastle upon Tyne, UK. |
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| Abstract: | A plasmid, pIF21, has been constructed that is able to donate transposons Tn1, Tn5 and Tn7. The transposons are located on a temperature-sensitive derivative of the incP1 plasmid pRP1, which is transferable to a wide range of Gram-negative genera. |
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