DNA of some anaerobic rumen fungi: G + C content determination |
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| Authors: | G. Billon-Grand,J.B. Fiol,A. Breton,A. Bruyè re,Z. Oulhaj |
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| Affiliation: | ICBMC, Section Levures, Université Lyon 1, Villeurbanne, France. |
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| Abstract: | The nuclear DNAs from five species of anaerobic rumen fungi have been isolated and purified by means of two extraction methods (with and without 8 M urea). Their G + C contents have been characterized by the thermal denaturation procedure of Marmur and Doty. As has already been shown in Neocallimastix frontalis, the results obtained by the two techniques demonstrated a very low G + C content (less than 20%) and the constant presence of satellite DNA. |
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| Keywords: | Anaerobic Rumen Fungi DNA G + C Content |
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