Iron and cadmium uptake by duodenum of hypotransferrinaemic mice |
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Authors: | Kishor B Raja Shahnaz E Jafri Timothy J Peters Robert J Simpson |
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Institution: | (1) Department of Clinical Biochemistry, King’s College London, London, UK;(2) Nutrition Sciences Research Division, King’s College London, London, UK;(3) Department of Biochemistry, King’s College London, Franklin Wilkins Building, Stamford St, London, SE1 9NN, UK |
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Abstract: | Absorption from food is an important route for entry of the toxic metal, cadmium, into the body. Both cadmium and iron are
believed to be taken up by duodenal enterocytes via the iron regulated, proton-coupled transporter, DMT1. This means that
cadmium uptake could be enhanced in conditions where iron absorption is increased. We measured pH dependent uptake of 109Cd and 59Fe by duodenum from mice with an in vitro method. Mice with experimental (hypoxia, iron deficiency) or hereditary (hypotransferrinaemia) increased iron absorption
were studied. All three groups of mice showed increased 59Fe uptake (p<0.05) compared to their respective controls. Hypotransferrinaemic and iron deficient mice exhibited an increase in 109Cd uptake (p<0.05). Cadmium uptake was not, however, increased by lowering the medium pH from 7.4 to 6. In contrast, 59Fe uptake (from 59FeNTA2) and ferric reductase activity was increased by lowering medium pH in control and iron deficient mice (p<0.05). The data show that duodenal cadmium uptake can be increased by hereditary iron overload conditions. The uptake is
not, however, altered by lowering medium pH suggesting that DMT1-independent uptake pathways may operate. |
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Keywords: | cadmium toxicity iron absorption iron deficiency iron overload |
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