Accuracy of cDNA microarray methods to detect small gene expression changes induced by neuregulin on breast epithelial cells |
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| Authors: | Bin?Yao,Sanjay?N?Rakhade,Qunfang?Li,Sharlin?Ahmed,Raul?Krauss,Sorin?Draghici,Jeffrey?A?Loeb mailto:jloeb@med.wayne.edu" title=" jloeb@med.wayne.edu" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author |
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| Affiliation: | (1) Center for Molecular Medicine and Genetics, Wayne State University, Detroit, Michigan, USA;(2) Department of Neurology, Wayne State University, Detroit, Michigan, USA;(3) Department of Computer Science, Wayne State University, Detroit, Michigan, USA;(4) Newton, Massachusetts, USA;(5) MCBI Wayne State Node, Wayne State University, Detroit, Michigan, USA |
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| Abstract: | ![]()
Background cDNA microarrays are a powerful means to screen for biologically relevant gene expression changes, but are often limited by their ability to detect small changes accurately due to "noise" from random and systematic errors. While experimental designs and statistical analysis methods have been proposed to reduce these errors, few studies have tested their accuracy and ability to identify small, but biologically important, changes. Here, we have compared two cDNA microarray experimental design methods with northern blot confirmation to reveal changes in gene expression that could contribute to the early antiproliferative effects of neuregulin on MCF10AT human breast epithelial cells. |
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