Expression pattern of two paralogs of the <Emphasis Type="Italic">PI</Emphasis>/<Emphasis Type="Italic">GLO</Emphasis>-like locus during <Emphasis Type="Italic">Orchis italica</Emphasis> (Orchidaceae,Orchidinae) flower development |
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Authors: | Marinella Salemme Maria Sica Luciano Gaudio Serena Aceto |
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Institution: | (1) Department of Biological Sciences, University of Naples Federico II, via Mezzocannone 8, 80134 Naples, Italy; |
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Abstract: | The class B MADS-box genes belong to two distinct functional groups: the AP3/DEF-like and the PI/GLO-like sub-families. In orchids, AP3/DEF-like genes are present in four copies, each with a different role in floral organ formation, which is described in the “orchid
code” model. Interestingly, the orchid PI/GLO-like genes are present in two copies in Orchidinae, whereas they are described as single copy in the other orchid lineages.
The two PI/GLO-like paralogs have site-specific different selective constraints; in addition, they show relaxation of purifying selection
when compared to the single-copy lineages. In this study, we present a comparative analysis of the expression patterns of
the two PI/GLO-like paralogs, OrcPI and OrcPI2, in floral tissues of Orchis italica in different developmental stages using real-time PCR. The two genes show similar expression profiles in the tissue examined,
with differences detectable between immature and mature inflorescence. In all cases, OrcPI2 is expressed at a higher level than OrcPI. Real-time PCR results reveal that the co-expression of the two duplicated loci could have a fully or partially redundant
function. The possible evolutionary fate of OrcPI and OrcPI2 is discussed as well as their involvement in ovary development. |
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