| 胡萝卜与川西獐牙菜不对称体细胞杂种的核/质基因组特征 |
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| 引用本文： | 李子东,邵菲,蔡云飞,向凤宁,夏光敏.胡萝卜与川西獐牙菜不对称体细胞杂种的核/质基因组特征[J].植物生理与分子生物学学报,2005,31(3):254-260. |
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| 作者姓名： | 李子东 邵菲 蔡云飞 向凤宁 夏光敏 |
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| 作者单位： | 1. 山东大学生命科学学院,济南,250100
2. 中国农业大学农业生物技术国家重点实验室,北京,100094 |
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| 基金项目： | 山东省自然科学基金，国家自然科学基金 |
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| 摘 要： | 胡萝卜(Daucus carota var.sativa)原生质体与经260μW/cm2紫外线照射的川西獐牙菜(Swertia mussotii)原生质体用PEG法诱导融合.对融合再生的克隆的5SrDNA间隔序列和RAPD分析结果得知,各再生克隆均存在双亲的核DNA及重组DNA.杂种的核基因组组成以胡萝卜(受体)为主,供体川西獐牙菜DNA谱带较少;UV照射剂量对体细胞杂种核基因组组成没有明显的影响.进一步对再生克隆叶绿体DNA的SSR分析表明,杂种细胞中双亲叶绿体基因组随机分离并发生重组.
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| 关 键 词： | 胡萝卜 川西獐牙菜 紫外线处理 不对称体细胞杂交 核/质基因组 |
| 修稿时间： | 2004年7月22日 |
The Characteristics of Nuclear/Cytoplasmic Genomes of Asymmetric Somatic Hybrid of Daucus carota var. Sativa and Swertia mussotii |
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| LI Zi-Dong,SHAO Fei,CAI Yun-Fei,XIANG Feng-Ning,XIA Guang-Min.The Characteristics of Nuclear/Cytoplasmic Genomes of Asymmetric Somatic Hybrid of Daucus carota var. Sativa and Swertia mussotii[J].Journal Of Plant Physiology and Molecular Biology,2005,31(3):254-260. |
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| Authors: | LI Zi-Dong SHAO Fei CAI Yun-Fei XIANG Feng-Ning XIA Guang-Min |
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| Institution: | School of Life Sciences, Shandong Univeristy, Jinan 250100, China. |
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| Abstract: | Protoplasts of Swertia mussotii irradiated by ultra-violet light (UV) 260 microW/cm(2) were fused with protoplasts of Daucus carota var. sativa using the PEG method. Analysis of 5S rDNA spacer sequences and random amplified polymorphic DNA (RAPD) showed the presence and combination of nuclear DNA from both parents. All hybrids mainly contained genomes of D. carota (receptor) RAPD bands, and a few donor RAPD bands of S. mussotii. There is no obvious relationship between the nuclear DNA composition was not affected by UV-dosage. SSR analysis of chloroplast DNA of the hybrid clones further revealed that cpDNAs of the parents were integrated and recombined in the hybrids randomly. |
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| Keywords: | Daucus carota var sativa Swertia mussotii UVtreatment asymmetric somatic hybridization nuclear andcytoplasmic genome |
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