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| 重离子诱变创制高产油微拟球藻新品种 | |
| 引用本文： | 王芝瑶,马玉彬,牟润芝,孙长江,张东远,王永飞.重离子诱变创制高产油微拟球藻新品种[J].生物工程学报,2013,29(1):119-122. |
| 作者姓名： | 王芝瑶 马玉彬 牟润芝 孙长江 张东远 王永飞 |
| 作者单位： | 1. 暨南大学生命科学技术学院生物工程学系,广东广州510632;中国科学院青岛生物能源与过程研究所生物燃料重点实验室,山东青岛266101 2. 中国科学院青岛生物能源与过程研究所生物燃料重点实验室,山东青岛,266101 3. 青岛首创瑞海水务有限公司,山东青岛,266042 4. 北京旭阳化工技术研究院有限公司,北京,100070 5. 暨南大学生命科学技术学院生物工程学系,广东广州,510632 |
| 基金项目： | 山东省自然科学基金 (No. ZR2011DQ007)，中国科学院知识创新工程重要方向项目 (No. KSCX2-EW-Q-22) 资助。 |
| 摘 要： | 以具有产业化前景的微拟球藻Nannochloropsis oceanica OZ-1为实验材料,利用碳重离子进行诱变育种,采用Imaging-PAM和酶标仪进行大规模筛选,最终获得两株高生长速率微拟球藻突变藻株(HP-1和HP-2),进一步分析显示两株突变藻株(HP-1和HP-2)生物量积累较野生型藻株大幅提高,在18d培养末期生物量分别提高了18％和26％,两株突变藻株油脂产率分别为295 mg/(L·d)和275 mg/(L·d),而野生型藻株为247 mg/(L·d).所获两株突变藻株生长速度快、油脂产率高,较野生型藻株优势明显. |
| 关 键 词： | 微拟球藻 重离子诱变 微藻生物柴油 |
| 收稿时间： | 2012/11/8 0:00:00 |
Improved lipid productivity of Nannochloropsis by heavy-ion irradiation mutagenesis |
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| Zhiyao Wang,Yubin M,Runzhi Mu,Changjiang Sun,Dongyuan Zhang and Yongfei Wang.Improved lipid productivity of Nannochloropsis by heavy-ion irradiation mutagenesis[J].Chinese Journal of Biotechnology,2013,29(1):119-122. | |
| Authors: | Zhiyao Wang Yubin M Runzhi Mu Changjiang Sun Dongyuan Zhang and Yongfei Wang |
| Institution: | Department of Biotechnology, School of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China; Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, Shandong, China;Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, Shandong, China;Qingdao Capital Group Water Technology Co., Ltd, Qingdao 266042, Shandong, China;Beijing Risun Chemical Industry Technology Research institute Co. Ltd, Beijing 100070, China;Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, Shandong, China;Department of Biotechnology, School of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China |
| Abstract: | Nannochloropsis has been considered as a promising feedstock for biodiesel production in recent years. To improve its lipid productivity, heavy-ion irradiation mutagenesis, an effectively breeding method used in plants and microorganisms was applied in Nannochloropsis oceanica OZ-1. After large-scale screening using Imaging-PAM and microplate-reader, two mutants (HP-1 and HP-2) with higher growth rate were isolated from the wild type N. oceanica. Subsequently analysis showed that after 18 days of cultivation biomass accumulation of the HP-1 and HP-2 mutant was increased by 19% and 26% respectively compare to the wild type. Total lipid productivity of the HP-1 and HP-2 mutant was 295 mg/(L·d) and 275 mg/(L·d), respectively, whereas that of the wild type was 247 mg/(L·d). Both mutants showed significantly advantage over their wild type concerning biomass accumulation and lipid productivity. |
| Keywords: | Nannochloropsis oceanica OZ-1 heavy-ion irradiation mutagenesis microalgal biodiesel |
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