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Noncompetitive enzyme immunoassay for the measurement of bronchial inhibitor in biological fluids
Authors:J M Tournier  J Jacquot  P Sadoul  J G Bieth
Affiliation:1. Inserm Unité 14, Plateau de Brabois, 54511 Vandoeuvre-les-Nancy, France;2. Inserm Unité 237, Faculté de Pharmacie, Université Louis Pasteur, 67048 Strasbourg, France
Abstract:
An enzyme-linked immunosorbent assay (ELISA) of bronchial inhibitor using rabbit antibronchial inhibitor antibody-coated polystyrene balls as the solid-phase antibody and peroxidase-labeled antibody as the conjugate is described. A crude antibody fraction is used for coating the solid phase. The assay can be run within 8 h and gives reproducible results in the range of 6 to 60 micrograms/l of bronchial inhibitor (mean within-run coefficient of variation, 7%). It can detect bronchial inhibitor concentrations as low as 2 micrograms/l (10(-10) M) and recovery of varying amounts of bronchial inhibitor added to bronchial liquids is greater than 90%. This enzyme immunoassay appears to be a convenient way to quantify bronchial inhibitor in biological fluids such as serum, sputum, or bronchoalveolar lavage fluid.
Keywords:To whom correspondence should be addressed.
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