Changes in nuclear content of protein conjugate histone H2A-ubiquitin during rooster spermatogenesis |
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Authors: | N Agell M Chiva C Mezquita |
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Institution: | Departamento de Fisiología, Facultad de Medicina, Universidad de Barcelona, Casanova 143, Barcelona-36, Spain |
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Abstract: | Electrophoretic analysis of acid-soluble chromosomal proteins isolated from rooster testis cell nuclei at different stages of spermatogenesis, revealed that the nuclear content of a protein identified by its solubility, electrophoretic mobility and amino acid analysis as the protein conjugate histone H2A-ubiquitin (uH2A, A24) changed markedly from meiotic cells to late spermatids. The protein was not detectable in tetraploid primary spermatocytes; it was present in 1.7% of the total amount of nucleosomal core histones in early spermatids and reached its maximum level (3.5% and 11%) at the end of spermiogenesis, when histones are replaced by the protamine galline. |
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Keywords: | Histone H2A Ubiquitin uH2A A24 Chromatin Spermatogenesis |
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